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KMID : 1137020080190040251
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Journal of Gynecologic Oncology
2008 Volume.19 No. 4 p.251 ~ p.255
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Comparison between L and E gene amplification analytical methods for human papillomavirus typing
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Cho Hong-Bum
Kim Kyung-Tai
Kim Young-Jae
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Abstract
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Objective: L and E6/E7 gene amplification analyses were compared to ide
Methods: L1 gene verified HPV typing was accomplished via polymerase chain reaction (PCR) and membrane assays. Verification of HPV typing via E6/E7 genes was accomplished through nested multiplexed PCR. The results from 104 samples were compared.
Results: The rates of accordance and difference were 35% and 65%, respectively. For 29% of the analyses, nested multiplexed PCR was more diversified than the membrane assay.
Conclusion: HPV can be classified into low-risk HPV and high-risk HPV groups. In parallel amplifications of the L and E genes is more efficient for accurate diagnosis in light of the different symptoms and attendant precautions of the risk groups.
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KEYWORD
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Human papillomavirus, L1 gene, E6/E7 gene
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